The authoritative guide on protein purification--now completely updated and revisedSince the Second Edition of Protein Purification was published in 1998, the sequencing of the human genome and other developments in bioscience have dramatically changed the landscape of protein research. This new edition addresses these developments, featuring a wealth of new topics and several chapters rewritten from scratch.Leading experts in the field cover all major biochemical separation methods for proteins in use today, providing professionals in biochemistry, organic chemistry, and analytical chemistry with quick access to the latest techniques. Entirely new or thoroughly revised content includes:High-resolution reversed-phase liquid chromatographyElectrophoresis in gelsConventional isoelectric focusing in gel slabs and capillaries and immobilized pH gradientsAffinity ligands from chemical and biological combinatorial librariesMembrane separationsRefolding of inclusion body proteins from E. coliPurification of PEGylated proteinsHigh throughput screening techniques in protein purificationThe history of protein chromatography
- 2011 John Wiley &amp; Sons
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