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Efficient Screening Techniques to Identify Mutants with TR4 Resistance in Banana: Protocols
Bananas are a staple food for over 500 million people and are also an important cash crop. Fusarium wilt, caused by the fungus Fusarium oxysporum f.sp. cubense, is one of the most destructive diseases of banana globally. Since the 1990s, an aggressive variant of this fungus, called Tropical Race 4 (... More
Language: ENGCopyright: 2022 -
Protocols for Pre-Field Screening of Mutants for Salt Tolerance in Rice, Wheat and Barley
by Souleymane Bado • Brian P. Forster • Abdelbagi M. A. Ghanim • Joanna Jankowicz-Cieslak • Günter Berthold • Liu LuxiangThis book offers effective, low-costand user-friendly protocols for the pre-field selection of salt-tolerant mutants in cereal crops. It presents simple methods for measuring soil salinity, includingsoil sampling and the analysis of water-soluble salts, and describes adetailed, but simple, screenin... More
Language: ENGCopyright: 2016 -
Biotechnologies for Plant Mutation Breeding: Protocols
This book is open access under a CC BY-NC 2. 5 license. This book offers 19 detailed protocols on the use of induced mutations in crop breeding and functional genomics studies, which cover topics including chemical and physical mutagenesis, phenotypic screening methods, traditional TILLING and TILL... More
Language: ENGCopyright: 2017 -
Low-Cost Methods for Molecular Characterization of Mutant Plants
by Joanna Jankowicz-Cieslak • Bradley J. Till • Owen A. Huynh • Mayada M. Beshir • Robert G. Laport • Bernhard J. HofingerThis book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for singl... More
Language: ENGCopyright: 2015